The Hymenoptera holdings of the Museum of Entomology (FSCA), are composed of approximately 600,000 prepared specimens, and rank as one of the larger collections in North America. The collection represents 2,509 genera, and 10,809 species. These holdings include 144 primary types, and 2,750 secondary types (see the Types Catalogue). The collection is worldwide in scope and has specimens from all faunal regions. Loans to researchers are welcome, and exchanges of specimens which would increase species representation within the collection are actively sought (exchange lists available).

Pinned and slide preserved specimens are housed in 3,051 insect drawers. An additional 540 drawers house the private Hymenoptera collections of C.C. Porter, and another 54 drawers house the Biological Control Collections of G.R. Buckingham, and the Biological Control Voucher Collection. 

The Hymenoptera collections are exceptionally well curated. Using a modified Smithsonian Collections Standards and Profiling System: 13% of the drawers are sorted and accessible, determined to superfamily and family level, 87% are determined to genera and species level (all identified and properly integrated; housed in soft bottom foam trays; unit tray header labels an drawer labels completed; genera and species lists completed).

Geographical strengths of the collection are for North America (particularly Florida, and the states of Arizona, California, Idaho, Maine, New Mexico, New York, North Carolina, Oklahoma, Oregon, Texas, and Utah), the Neotropics (particularly Argentina, Bahamas, Bolivia, Chile, Colombia, Dominican Republic, Ecuador, Mexico, Peru, Trinidad, and Venezuela), Africa (Egypt, Saudi Arabia, South Africa, and Tunisia), and tropical Asia (Taiwan). Suprafamily and family strengths are Symphyta (including the H.N. Greenbaum collection), Ichneumonidae (including parts of the C.C. Porter and G.H. Heinrich collections), Aphelinidae (including parts of the G.A. Evans collection), Encyrtidae (including the F.D. Bennett collection), Eumenidae (including the L.A. Stange Zethinae collection), Formicidae, Sphecidae, and Apidae (including the L.A. Stange Anthidiinae collection).